Direct Observation of a Defect-Mediated Viscoelastic Transition in a Hydrogel of Lipid Membranes and Polymer Lipids

We present the first direct imaging of a new hydrogel of lipid membranes containing polymer lipids. Freeze-fracture electron microscopy shows unambiguously that the hydrogel’s surprisingly large viscoelasticity is explained by a novel defect topology of interconnections between defects. The defects are spherulites with high membrane curvatures which are either isotropic or cylinderlike. A lower concentration of dislocation-type defects was also observed. The interconnections between the defects distinguish the hydrogel from simple “onion” phases of multilamellar vesicles with a smaller viscoelasticity. [S0031-9007(97)03388-7]